目的:探讨靶向高通量测序(tNGS)在疑似肺炎患者中的诊断价值。方法:回顾性分析335例疑似肺炎患者的病例资料,所有患者均采集下呼吸道标本(首选肺泡灌洗液)并进行了tNGS检测和传统病原学检测(CMTs)。以综合诊断为标准分析tNGS和CMTs在疑似肺炎患者中的诊断效能,比较两者所检出的致病病原体分布以及在不同类型病原体中的诊断效能。结果:335名患者中,34名患者(10.1%)被诊断为非感染性病因,301名(89.9%)被诊断为感染性肺炎,其中270名患者被诊断为微生物学确诊的肺炎。以综合诊断为标准,tNGS的总体诊断敏感性(81.1% vs. 38.9%, P Objective: To explore the diagnostic value of targeted next-generation sequencing (tNGS) in patients with suspected pneumonia. Method: A retrospective analysis was conducted on the medical records of 335 patients with suspected pneumonia. All patients underwent collection of lower respiratory tract specimens (preferably bronchoalveolar lavage fluid), followed by testing with targeted next-generation sequencing (tNGS) and conventional microbiological tests (CMTs). Using a comprehensive diagnostic standard, the diagnostic performance of tNGS and CMTs in patients with suspected pneumonia was evaluated. The distribution of pathogenic microorganisms detected by both methods and their diagnostic efficacy for different types of pathogens were compared. Result: Among the 335 patients, 34 (10.1%) were diagnosed with non-infectious causes, and 301 (89.9%) were diagnosed with infectious pneumonia, with 270 cases confirmed microbiologically. Based on the comprehensive diagnostic standard, tNGS exhibited significantly higher overall diagnostic sensitivity (81.1% vs. 38.9%, P Aspergillus and Mycobacterium tuberculosis. Conclusion: tNGS demonstrates a broader range of pathogen identification and higher sensitivity and accuracy in the diagnosis of suspected pneumonia patients. tNGS can complement traditional microbiological testing and provide effective guid
